Ortiz-Espín, A.; Morel, E.; Juarranz, Á.; Guerrero, A.; González, S.; Jiménez, A.; Sevilla, F. An Extract from the Plant Deschampsia antarctica Protects Fibroblasts from Senescence Induced by Hydrogen Peroxide. Both percentages of positive cells and mean fluorescent intensity for specific antibodies were determined by using the Image J analysis software. and M.M. The statements, opinions and data contained in the journals are solely You seem to have javascript disabled. Please note that many of the page functionalities won't work as expected without javascript enabled. Official CosIng Information Products with Deschampsia Antarctica Leaf Extract. Recommendation for deschampsia antarctica leaf extract flavor usage levels up to: not for flavor use. Phototoxicity from ultraviolet ionizing radiations is a permanent environmental aggression to skin, and a direct cause of skin aging and tumorigenesis. 20, no. In vitro studies have shown that DA aqueous extract (DAE) can down-regulate the pollutant-induced AhR activation [13]. Subscribe to receive issue release notifications and newsletters from MDPI journals, You can make submissions to other journals. Extract of Deschampsia antarctica (EDA) Prevents Dermal Cell Damage Induced by UV Radiation and 2,3,7,8-Tetrachlorodibenzo-p-dioxin March 2019 International Journal of Molecular Sciences 20(6):1356 ; Budanov, A.; He, Y.Y. Fragrance ingredients in hair permanent and other hair chemical treatments have been placed in Category 7. ; Ruhland, C.T. Afterwards, coverslips were washed in PBS and then incubated with specific anti-IgG secondary antibodies coupled to AlexaFluor488 or AlexaFluor546 (Invitrogen, Waltham, MA, USA). Briefly, dry green leaves, harvested from cultured Deschampsia antarctica plants in defined conditions, were milled and extracted by percolation with water at 40–60 °C, during 4–6 h. The extract was filtered through a 1μm filter and … Bennett, B.T. 25 μg of total protein or 15 μg of cytoplasmic or nuclear extract per total sample was loaded, respectively. Distinct Role of Sesn2 in Response to UVB-Induced DNA Damage and UVA-Induced Oxidative Stress in Melanocytes. Loricrin is a protein which reports healthy differentiation of keratinocytes. S.G. has a role of consultor for Cantabria Labs, A.P-D. belongs to Research and Development Department at Cantabria Labs, The statements, opinions and data contained in the journal, © 1996-2020 MDPI (Basel, Switzerland) unless otherwise stated. Recommendation for deschampsia antarctica leaf extract usage levels up to: not for fragrance use. High throughput sequencing showed that up to 92% of DNA reads, and 68% of RNA reads, in soils from the islands were accounted for by the fungus. (Angiosperm: family Graminaceae) plants were collected from Robert Island, South Shetland Islands, Maritime Antarctic, during February, 1992 and January, 1993, and were extracted with 80% ethanol. Immunofluorescence imaging of DNA damage response proteins: Optimizing protocols for super-resolution microscopy. The Antarctic hair grass, Matrix metalloproteinases (MMPs) constitute a heterogeneous family of enzymes capable of hydrolyzing collagen and degrading different components of the ECM, and are involved in several physiological and pathological processes. Kawai, C.; Araújo-Chaves, J.C.; Magrini, T.; Sanches, C.O. We use cookies on our website to ensure you get the best experience. ; Grindel, A.; Hübenthal, U.; Brenden, H.; Grether-Beck, S.; Vielhaber, G.; Johncock, W.; Krutmann, J.; et al. Kohler, H.; Contreras, R.A.; Pizarro, M.; Cortes-Antiquera, R.; Zuniga, G.E. This research was funded by Industrial Farmacéutica Cantabria and by the Spanish grant from Instituto de Salud Carlos III MINECO and Feder Funds (FIS PI18/00708). those of the individual authors and contributors and not of the publisher and the editor(s). Briefly, dry green leaves, harvested from cultured Deschampsia antarctica plants in defined conditions, were milled and extracted by percolation with water at 40-60[degrees]C, during 4 … ; Grace, J.R. Predictability of physicochemical properties of polychlorinated dibenzo-p-dioxins (PCDDs) based on single-molecular descriptor models. ; Visualization, A.Z. Although the precise mechanisms at play will require extensive mechanistic characterization, beyond the scope of this report, it is clear that certain mechanisms sensing and transducing the impact of UVB on the cell are active despite the presence of EDA. UVRAG: A new player in autophagy and tumor cell growth. J. Mol. Banyasz, A.; Martinez-Fernandez, L.; Balty, C.; Perron, M.; Douki, T.; Improta, R.; Markovitsi, D. Absorption of Low-Energy UV Radiation by Human Telomere G-Quadruplexes Generates Long-Lived Guanine Radical Cations. Deschampsia antarctica extract (EDAFENCE® hereinafter called EDA) was obtained from IFC, Madrid, Spain. Zamarrón, A.; Morel, E.; Lucena, S.R. 1% Triton X-100 in PBS before incubation with specific primary antibodies for γH2A.X (Cell Signaling, Danvers, MA, USA) (dilution 1:500), active caspase-3 (Cell Signaling) (dilution 1:500), LC3A/B (Abcam) (dilution 1:100), Survivin (Abcam, Cambridge, MA, USA) (dilution 1:500), AhR (ThermoFisher Scientific, Waltham, MA, USA) (dilution 1:100) and Loricrin (Sigma) (dilution 1:100). MMP1 was analysed in supernatants from cultures of HDF treated with 0.1 or 0.3 mg/mL EDA for 24 h and then irradiated with UVB light at different dose: 20 and 40 mJ/cm. Pajares, M.; Cuadrado, A.; Rojo, A.I. Differences were considered to be significant when. and M.M. The methods for obtaining the extract have been previously published . Subscribe to receive issue release notifications and newsletters from MDPI journals, You can make submissions to other journals. ; Salvesen, G.S. Linden, J.; Lensu, S.; Tuomisto, J.; Pohjanvirta, R. Dioxins, the aryl hydrocarbon receptor and the central regulation of energy balance. Our observations support that EDA is an effective antitoxicity compound protecting from major environmental pollutants such as dioxins, independently from its photoprotective activity. Different concentrations of TCDD (15–300 nM) were tested on human dermal fibroblasts and keratinocytes at different incubation times, always without exceeding a final DMSO concentration of 0.1% (Sigma, Saint Louis, MO, USA). In this work, antioxidant physiology of D. antarctica was studied in response to UVB induced oxidative changes. Because toxicity by environmental pollutants is a major aspect of skin damage, aging and tumorigenesis, we decided to explore whether EDA additionally confers protection from the toxic effects of a prototypical environmental pollutant: TCDD, the most common dioxin and a potent tissue damaging agent [, Exposure to either HDF and HaCaT cells to nanomolar concentrations of TCDD elicited an upregulation of AhR protein (the natural ligand) at times as early as 90min as assessed by epifluorescence microscopy (, Finally, we assessed the functional impact of abrogating TCDD exposure-associated signalling upon EDA supplementation. Finally, coverslips were mounted on slides using ProLong™-DAPI (Life Technologies, Waltham, MA, USA) for detection of the nuclei and analysed by fluorescence microscopy (Olimpus BX61). photoaging; ultraviolet light; dioxins; natural extract; in vitro studies; dermal fibroblast; keratinocytes, Photoinduced Phenomena in Nucleic Acids II: DNA Fragments and Phenomenological Aspects, Help us to further improve by taking part in this short 5 minute survey, Decreased Protein Kinase C-β Type II Associated with the Prominent Endotoxin Exhaustion in the Macrophage of FcGRIIb−/− Lupus Prone Mice is Revealed by Phosphoproteomic Analysis, Molecular Toxicological Mechanisms of Synthetic Cathinones on C2C12 Myoblasts, The Role of Tocotrienol in Preventing Male Osteoporosis—A Review of Current Evidence, Mitotic Catastrophe Induced in HeLa Tumor Cells by Photodynamic Therapy with Methyl-aminolevulinate, https://www.mdpi.com/1422-0067/20/6/1356/s1, http://creativecommons.org/licenses/by/4.0/, Inhibitors the tissue inhibitor of metalloproteinases. Exposure of all tested cell types to EDA blunted hallmarks of UVR-induced canonical DNA damage responses and downstream stress/proapoptotic signalling, such as autophagy, caspase activation and MMP1 secretion. To understand how the plants survive freezing temperatures year-round, antifreeze activity was assayed in apoplastic extracts obtained from both non-acclimated and cold … Our observations support the potential of EDA as a supplement for the pharmacological protection of skin health against ionizing radiation and chemical damage-associated protumoral insult and aging. Day, T.A. Rothhammer, V.; Quintana, F.J. For the detection of total AhR, Tubulin, PARP and β-actin, extracts were obtained by using the Bio-world Protein Extraction Buffer with phosphatase inhibitors (Roche, Basel, Switzerland) and proteases (Roche), following the manufacturer’s instructions. ; Xiong, F.S. 2.1. Deschampsia antartica (DA) is a polyextremophile plant native to Antarctica . Statistical significance was determined by statistical test of analysis of variance (ANOVA) or two-way ANOVA and Bonferroni post hoc tests, using GraphPad Prism 5.00 (GraphPad Software, Inc., San Diego, CA, USA). They contribute to the regulation of cell growth, inflammation or angiogenesis by modulating cell signalling; and to the establishment of a specific tumour microenvironment through stromal remodelling. See further details. photoaging; ultraviolet light; dioxins; natural extract; in vitro studies; dermal fibroblast; keratinocytes, Help us to further improve by taking part in this short 5 minute survey, Decreased Protein Kinase C-β Type II Associated with the Prominent Endotoxin Exhaustion in the Macrophage of FcGRIIb−/− Lupus Prone Mice is Revealed by Phosphoproteomic Analysis, Molecular Toxicological Mechanisms of Synthetic Cathinones on C2C12 Myoblasts, The Role of Tocotrienol in Preventing Male Osteoporosis—A Review of Current Evidence, Mitotic Catastrophe Induced in HeLa Tumor Cells by Photodynamic Therapy with Methyl-aminolevulinate. Aqueous extract ( EDAFENCE® hereinafter called EDA ) was obtained from IFC, Madrid, Spain UV. Deschampsia antartica ( DA ) is a polyextremophile plant native to antarctica [ ]!, S.R extract was obtained from Cantabria Labs, Madrid, Spain ) is the component... Vogel, C.F and function Project administration, S.G. ; Writing – review & editing, E.M.,,! 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